Expanding the natural diversity of microbial Exopolysaccharides (EPS) by screening and genetic engineering approaches. Dr.-Ing. Jochen Schmid. Technical University of Munich

2018-06-13 12:00
2018-06-13 13:30
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Summary:
Microbial exopolysaccharides represent a valuable class of biodegradable biopolymers, which can be used in many applications. Based on their high structural diversity they are very promising substances for tailoring the specific properties within the targeted applications. Up to now, only a limited number of microbial exopolysaccharides is used commercially, even when nearly 50 % of all bacteria can produce at least one polysaccharide. This fact is based on the limitations of the screening technologies normally used for the identification of novel exopolysaccharide producers. Additionally, the genetic engineering of novel wild type isolates is often time consuming and tedious due to the need of establishing specific appropriate molecular tools. This talk will give an overview of novel screening techniques as well as genetic engineering approaches towards expanding the pool of microbial exopolysaccharides. By use of synthetic biology approaches further insights in the function of the biosynthesis pathways as well as characterization of single genes involved in the assembly of bacterial exopolysaccharides can be realized. Finally, characterization of variants designed by genetic engineering approaches will be presented.